Replacing immunoassays with tryptic digestion-peptide immunoaffinity enrichment and LC–MS/MS
نویسندگان
چکیده
منابع مشابه
Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
There is a great need for quantitative assays in measuring proteins. Traditional sandwich immunoassays, largely considered the gold standard in quantitation, are associated with a high cost, long lead time, and are fraught with drawbacks (e.g. heterophilic antibodies, autoantibody interference, 'hook-effect').(1) An alternative technique is affinity enrichment of peptides coupled with quantitat...
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BACKGROUND Quantification of serum tumor markers plays an important role in determining whether patients treated for cancer require further therapy. Whereas large-scale proteomic efforts aim to identify novel tumor markers to facilitate early detection, optimization of methods for quantifying known tumor markers offers another approach to improving management of malignancies. For example, immun...
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The peptides produced by the tryptic digestion of S-aminoethylated bovine pancreatic ribonuclease support the generally accepted amino acid sequence for this enzyme and also demonstrate the usefulness as well as some special limitations of the aminoethylation procedure. The relative extents of hydrolysis at different peptide bonds were estimated from the yields of purified peptides. Under the c...
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New approaches to rapid examination of proteins and peptides in complex food matrices are of great interest to the community of food scientists. The aim of the study is to examine the influence of microwave irradiation on the acceleration of enzymatic cleavage and enzymatic digestion of denatured proteins in cooked meat of five species (cattle, horse, pig, chicken and turkey) and processed meat...
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Immunoaffinity enrichment of proteotypic peptides, coupled with selected reaction monitoring, enables indirect protein quantification. However the lack of suitable antibodies limits its widespread application. We developed a method in which multi-specific antibodies are used to enrich groups of peptides, thus facilitating multiplexed quantitative protein assays. We tested this strategy in a pha...
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ژورنال
عنوان ژورنال: Bioanalysis
سال: 2012
ISSN: 1757-6180,1757-6199
DOI: 10.4155/bio.11.319